| 货号 | FAB5196T-100UG |
| 别名 | AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse Notch-2 in direct ELISAs. In direct ELISAs, 50-100%cross-reactivity with recombinant human (rh) Notch-2 and no cross-reactivitywith rhNotch-1, -2, -3, recombinant mouse (rm) Notch-1, rmNotch-3, recombinantrat (rr) Notch2, or rrDLL1 is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4853 (Human); 29492 (Rat) |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse Notch-2 Leu26-Val528 (predicted) Accession # O35516 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 594 |
| 背景 | Notch-2 (also Motch-2) is a 280-300 kDa member of the notch family of transmembrane (TM) proteins. It is found on multiple cell types and exhibits multiple functions. On CD8+ T cells, it augments cytotoxic activity; on pre-B cells, it drives B1 B cell development; on endothelial cells, it induces apoptosis; and on proerythroblasts, it promotes proliferation. Mature mouse Notch-2 is a 2445 amino acid (aa) type I TM glycoprotein. It undergoes Golgi processing to generate a 180-200 kDa extracellular domain (ECD) (aa 26-1606) possibly covalently-linked to a 100-110 kDa membrane bound C-terminal segment (aa 1607-2470). Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellulardomain) that serves as a potential nuclear transcription factor. There are three potential splice variants. One shows a 32 aa substitution for aa 293-2470, a second contains a 13 aa substitution for aa 1-1954, and a third possesses a five aa substitution for aa 224-227 coupled to an 11 aa substitution for aa 1704-1713. Over aa 26-528, mouse Notch-2 shares 96% and 94% aa identity with rat and human Notch-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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