| 货号 | IC7428N-100UG |
| 别名 | EC 1.8.1; EC 1.8.1.9; Gene associated with retinoic and IFN-induced mortality 12 protein; Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM12; GRIM-12; GRIM-12TR1; KDRF; KM-102-derived reductase-like factor; MGC9145; oxidoreductase; Thioredoxin Reductase 1; thioredoxin reductase 1, cytoplasmic; thioredoxin reductase GRIM-12; Thioredoxin reductase TR1; TR; TR1; TRXR1; TRXRD1; TXNR; TXNRD1; TXNRTRXR1 |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects mouse Thioredoxin Reductase 1/TRXR1 in direct ELISAs and human, mouse, and rat Thioredoxin Reductase 1/TRXR1 in Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7296 (Human); 50493 (Mouse); 58819 (Rat) |
| 免疫原 | E. coli-derived recombinant mouse Thioredoxin Reductase 1/TRXR1 Met1-Ile497 Accession # Q16881 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Alexa Fluor 700 |
| 背景 | Thioredoxin reductase 1 (TRXR1) is an approximately 70 kDa member of the class-I pyridine nucleotide-disulfide oxidoreductase family. Human TRXR1 is 649 amino acids (aa) in length. Residues 151-152 constitute a propeptide that is deleted from the mature protein. Splicing variants produce five additional isoforms for human TRXR1. Isoform 2 has a 32 aa substitution for aa 107-138 and a deletion of aa 1-106. Isoform 3 has a deletion of aa 1-51 and a 49 aa substitution for aa 52-100. Isoform 4 is missing aa 1-98 and has a 3 aa substitution for aa 99-101. Isoform 5 has a deletion of aa 1-150. Residues 56-156 make up a glutaredoxin domain, and residues 520-632 constitute a pyridine nucleotide-disulphide oxidoreductase dimerization domain. In addition, there are three phosphotyrosines at positions 161, 163, and 281, and a selenocysteine at position 648. Human TRXR1 shares 74% and 70% aa sequence identity with mouse and rat TRXR1, respectively. Isoform 1 is involved in glutaredoxin activity as well as thioredoxin reductase activity, and it induces actin and tubulin polymerization, which leads to formation of cell membrane protrusions. Isoform 4 has been shown to enhance the transcriptional activity of the beta receptor only. Finally, isoform 5 mediates cell death induced by a combination of interferon-beta and retinoic acid. Isoform 1 is expressed mostly in the Leydig cells, but also in the ovary, spleen, heart, liver, kidney, and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in the kidney, uterus, testis, ovary, prostate, placenta, and fetal liver. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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