| 货号 | FAB5486A |
| 别名 | FLJ11751; FLJ30634; KIAA0463plexin 2; OCT; OCTSemaphorin receptor OCT; plexin A2; PLXN2; PLXN2plexin-A2; PLXNA2; transmembrane protein OCT |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse and rat Plexin A2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A3, and recombinant human Plexin B1 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5362 (Human); 18845 (Mouse); 289392 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin A2 Met35-Pro1237 Accession # P70207 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Allophycocyanin |
| 背景 | Plexin A2 (also OCT) is a 230-250 kDa member of the plexin-A subfamily, plexin family of proteins. It is found on cerebellar granule cells, sensory neurons, CD31+LYVE+ lymphoid endothelial cells, osteoblasts, and cardiac neural crest cells. It blocks neurite outgrowth and lymphatic capillary formation, but promotes cell migration and osteogenesis, and does so by serving as a receptor for Sema6A and B, and as a coreceptor with neuropilins for Sema3A and C. Notably, it would appear to contribute to the receptor complex for BMPs by interacting with both BMPR1A/ALK3 and BMPR1B/ALK6 in conjunction with BMPR2. Mature mouse Plexin A2 is an 1860 amino acid (aa) type I transmembrane homodimeric glycoprotein. It contains a 1203 aa extracellular domain (ECD) and a 636 aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508), three PSI domains (aa 510-855) and four IPT regions (aa 858-1228). Over aa 35-1237, mouse Plexin A2 shares 96% aa sequence identity with human Plexin A2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Plexin A2 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of Plexin A2 in PC‑12 Rat Cell Line by Flow Cytometry. PC‑12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of Plexin A2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins. |
