货号 | FAB3964A |
别名 | EDG8; Edg-8; EDG8S1P receptor Edg-8; Endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; LPB4; S1P receptor 5; S1P5; S1PR5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8; Sphingosine 1-phosphate receptor Edg-8; sphingosine-1-phosphate receptor 5; SPPR-1; SPPR-2 | 全称 | Endothelial Differentiation Gene 8 |
反应种属 | Human |
应用 | Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects human and mouse S1P5/EDG‑8. Stains human and mouse S1P5/EDG‑8 transfectants but not irrelevant transfectants. |
使用方法 | Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 53637 (Human); 94226 (Mouse); 60399 (Rat) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | BaF3 mouse pro-B cell line transfected with human S1P5/EDG‑8 Met1-Asp398 Accession # Q9H228 |
生物活性 | Human, Mouse |
标记 | Allophycocyanin |
背景 | S1P5 is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P5 is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P5 show 96% and 97% aa identity with mouse and rat S1P5, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P5 is upregulated in large granular lymphocytic leukemias. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human S1P5/EDG-8 and eGFP was stained with and either (A) Mouse Anti-Human S1P5/EDG‑8 APC‑conjugated Monoclonal Antibody (Catalog # FAB3964A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins. | |
Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP was stained with and either (A) Mouse Anti-Human S1P5/EDG‑8 APC‑conjugated Monoclonal Antibody (Catalog # FAB3964A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins. |