| 货号 | FAB1277A |
| 别名 | AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; jagged 1; Jagged1; JAGL1; protein jagged-1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human, mouse, and rat Jagged 1. In direct ELISAs, no cross‑reactivity with recombinant human Jagged 2 is observed. Stains human Jagged 1 transfectants but not the parental cell line or human Jagged 2 transfectants in flow cytometry. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 182 (Human); 29146 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0-derived recombinant human Jagged 1 Ser32-Asp296 Accession # P78504 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Allophycocyanin |
| 背景 | Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97% and 96% aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Jagged 1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human/Mouse/Rat Jagged 1 APC‑conjugated Monoclonal Antibody (Catalog # FAB1277A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of Jagged 1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse Anti-Human/Mouse/Rat Jagged 1 APC‑conjugated Monoclonal Antibody (Catalog # FAB1277A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins. |
