Human/Mouse IL-12/IL-35 p35 Allophycocyanin MAb (Cl 27537) (100 TESTS)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human/Mouse IL-12/IL-35 p35 Allophycocyanin MAb (Cl 27537) (100 TESTS)

货号： IC2191A 基本售价： 5086.1 元规格： 100 Tests

产品信息

概述
货号	IC2191A
别名	IL-12A
全称	Interleukin 12 p35
反应种属	Human/Mouse
应用	Intracellular Staining by Flow Cytometry
目标/特异性	Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection. Authors: Wang, Jia M, Shi, Lei, Ma, Cheng J, Ji, Xiao J, Ying, Ruo S, Wu, Xiao Y, Wang, Ke S, Li, Guangyu, Moorman, Jonathan, Yao, Zhi Q J Virol, 2013;87(8):4372-83. Species: Human Sample Type: Whole Cells Application: Flow Regulatory T cells that recognize a ubiquitous stress-inducible self-antigen are long-lived suppressors of autoimmune arthritis. Authors: van Herwijnen M, Wieten L, van der Zee R, van Kooten P, Wagenaar-Hilbers J, Hoek A, den Braber I, Anderton S, Singh M, Meiring H, van Els C, van Eden W, Broere F Proc Natl Acad Sci U S A, 2012;109(35):14134-9. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35 Arg23-Ser219 Accession # P29459
生物活性	Human, Mouse
标记	Allophycocyanin
背景	p35is a 34-36 kDa monomeric member of the IL-6 superfamily of molecules. It is 219 amino acids in length, and possiblycontains an N-terminal 22 aa signal sequence. In any event, it does not appear to be individually secreted, and itsrelease from cells is commonly assumed to be as part of a disulfide-linkedheterodimer linked to either p40 (forming IL-12) or EBI3 (forming IL-35). Multiple cell types are known to express p35,and include keratinocytes, CD138⁺ plasma cells, naïve B cells, monocyte-deriveddendritic cells, macrophages, CD86^loB7-H1^hi CD206+B7-DC+ tolerogenic dendritic cells, neutrophils, activated monocytes andmacrophages, trophoblasts, testicular endothelial cells, and T cells, includingCD4⁺ and CD8⁺ T helper cells, and CD4⁺CD25⁺ Tregs. The presence of the subunit does notnecessarily indicate function, as the p35:p40 heterodimer is associated withpromoting inflammatory IFN-gamma production by NK cells, while the p35:EBI3heterodimer is anti-inflammatory through its inhibition of Th17 celldevelopment. Over aa 23-219, human p35shares 83% and 57% aa sequence identity with pig and mouse p35, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Markers Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis ICC / IHC Images: IL-12/IL-35 p35 IL-10 and IL-12 Influence in Dendritic Cell Maturation IL-12 Family IL-12: Cell Protection from UVB Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th1 Cells

参考图片

Detection of IL‑12/IL‑35 p35 in Human PBMCs or Mouse Splenocytes by Flow Cytometry. (A) Human peripheral blood mononuclear cell (PBMCs) or (B) mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 APC‑conjugated Monoclonal Antibody (Catalog # IC2191A) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.