| 货号 | FAB7005A |
| 别名 | 4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse VISTA/B7-H5/PD-1H in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human VISTA/B7-H5/PD-1H is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64115 (Human); 74048 (Mouse); 690899 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse VISTA/B7-H5/PD-1H Phe33-Ala191 Accession # Q9D659 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | VISTA, also known as B7-H5, PD-1H, Platelet Receptor Gi24, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, VISTA appears to positively interact with BMP4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, VISTA both promotes MT1-MMP expression and activity, and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature mouse VISTA is a 277 amino acid (aa) type I transmembrane glycoprotein (aa 33-309). It contains a 149 aa extracellular region (aa 33-191) with one V-type Ig-like domain (aa 33-161) and a 97 aa cytoplasmic domain. Based on human VISTA, mouse Gi24 will likely undergo proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment, plus a 25-30 kDa membrane-bound fragment. There are two potential isoform variants. One contains a deletion of aa 127-187, while another shows an alternative start site at Met82. Over aa 33-191, mouse VISTA/Dies1 shares 78% and 70% aa sequence identity with rat and human VISTA, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of VISTA/B7-H5/PD-1H in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Sheep Anti-Mouse VISTA/B7-H5/PD-1H APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7005A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins. | |
Detection of VISTA/B7-H5/PD-1H in HEK293 Human Cell Line Transfected with Mouse VISTA and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse VISTA or (B) irrelevant transfectants and eGFP was stained with Sheep Anti-Mouse VISTA/B7-H5/PD-1H APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7005A). Quadrant markers were set based on control antibody staining (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins. |
