Mouse/Rat DARC Phycoerythrin Affinity Purified PAb (100 TESTS)【科瑞奥博】

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Mouse/Rat DARC Phycoerythrin Affinity Purified PAb (100 TESTS)

货号： FAB6695P 基本售价： 4947.3 元规格： 100 Tests

产品信息

概述
货号	FAB6695P
别名	ACKR1; CCBP1; CD234 antigen; CD234; Dfy; Duffy Antigen; Duffy antigen/chemokine receptor; Duffy blood group antigen; Duffy blood group, chemokine receptor; Fy Antigen; Fy glycoprotein; FYDuffy blood group; Glycoprotein D; GPD; GPDWBCQ1; GpFy; Plasmodium vivax receptor
全称	Duffy Antigen, Receptor For Chemokines
反应种属	Mouse/Rat
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects mouse and rat DARC in Western blots.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2532 (Human); 13349 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Atypical chemokine receptor 1 on nucleated erythroid cells regulates hematopoiesis Authors: J Duchene, I Novitzky-B, A Thiriot, M Casanova-A, M Bianchini, SL Etheridge, E Hub, K Nitz, K Artinger, K Eller, J Caamaño, T Rülicke, P Moss, RTA Megens, UH von Andria, A Hidalgo, C Weber, A Rot Nat. Immunol., 2017;0(0):. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse DARC Met1-Pro61, Ala115-Cys127, Ser186-Lys205, Tyr264-Asn285 Accession # NP_034175
生物活性	Mouse, Rat
标记	Phycoerythrin
背景	DARC (Duffy Antigen Receptor for Chemokines; also CD234) is a 40-46 kDa glycoprotein member of the Duffy family of silent heptahelical chemokine receptors. It is expressed in liver and on select neurons, erythrocytes and the endothelium of postcapillary venules. Unlike traditional chemokine receptors, DARC cannot signal through G-proteins as it lacks a DRYLAIVHA cytoplasmic motif. DARC has three potential functions: first, it binds circulating inflammatory-type chemokines, serving as a repository for future chemokine release; second, it acts as a vehicle by which chemokines are transported from the abluminal to the luminal side of endothelium; and third, it complexes with signal-transducing chemokine receptors to create a nonsignaling heterodimer. Mouse DARC is 334 amino acids (aa) in length. It contains a 62 aa N-terminal extracellular region, and a 28 aa C-terminal cytoplasmic tail. There is one potential splice variant that shows a 42 aa substitution for aa 133-334. Collectively, over the four extracellular domains (aa 1-62, 115-127, 186-205, 264-285), mouse DARC shares 52% and 75% aa identity with human and rat DARC, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	CC Chemokines and Receptors Chemokine Receptors Chemokines and Receptors in Angiogenesis Human CD Marker Products Red Blood Cells (RBCs)

参考图片

Detection of DARC in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse TER‑119 APC‑conjugated Monoclonal Antibody (Catalog # FAB1125A) and either (A) Sheep Anti-Mouse/Rat DARC PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6695P) or (B) Normal Sheep IgG Phycoerythrin Control (Catalog # IC016P). View our protocol for Staining Membrane-associated Proteins.

Detection of DARC in HEK293 Human Cell Line Transfected with Mouse DARC and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse DARC and eGFP was stained with and either (A) Sheep Anti-Mouse/Rat DARC PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6695P) or (B) Normal Sheep IgG Phycoerythrin Control (Catalog # IC016P). View our protocol for Staining Membrane-associated Proteins.