| 货号 | FAB17291N |
| 别名 | Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2 | 全称 | Triggering Receptor Expressed on Myeloid Cells 2 |
| 反应种属 | Human/Mouse |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human and mouse TREM-2 in direct ELISAs and Western blots. Stains TREM-2 transfectants but not TREM-1 transfectants. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 54209 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse TREM-2 extracellular domain Accession # Q99NH8 |
| 生物活性 | Human, Mouse |
| 标记 | Alexa Fluor 700 |
| 背景 | TREM-2 is a single Ig domain receptor expressed by cells of myeloid lineage. Two closely related transcripts were reported in mouse and designated TREM-2a and TREM-2b. TREM-2b is a pattern recognition receptor that binds anionic ligands. TREM-2 associates with the adapter protein DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of TREM‑2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM‑2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB17291N, filled histogram) or isotype control antibody (Catalog # IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of TREM‑2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human/Mouse TREM‑2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB17291N, filled histogram) or isotype control antibody (Catalog # IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of TREM‑2 in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Rat Anti-Human/Mouse TREM‑2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB17291N, filled histogram) or isotype control antibody (Catalog # IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of TREM‑2 in HEK Human Cell Line Transfected with Human TREM-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TREM-2 or (B) human TREM-1 and eGFP was stained with Rat Anti-Human/Mouse TREM‑2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB17291N). Quadrant markers were set based on control antibody staining (Catalog # IC013N). View our protocol for Staining Membrane-associated Proteins. |
