| 货号 | FAB7050A |
| 别名 | CD323; JAM-2; JAM3; JAMC; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C | 全称 | Junctional Adhesion Molecule C |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse JAM-C (CD323) in flow cytometry. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 83700 (Human); 83964 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Function of Jam-B/Jam-C interaction in homing and mobilization of human and mouse hematopoietic stem and progenitor cells. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse JAM‑C (CD323) Val32-Asn241 Accession # Q9D8B7 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | JAM-C (Junctional Adhesion Molecule-C), also known as JAM-3 and JAM-2, is a 40-45 kDa member ofthe JAM family, IgSF of molecules. It isa type I transmembrane glycoprotein that is further classified as a classicalJAM with a short cytoplasmic tail vs. non-classical JAMs that contain longcytoplasmic tails. Mature mouse JAM-C is289 amino acids (aa) in length, and contains a 212 aa extracellularregion. This region possesses oneN-terminal V-type and one C2-type Ig-like domain (aa 30-241). In human, JAM-C is perhaps best known as acomponent of the epithelial cell tight junction. In concert with two other transmembraneprotein types (claudins and occludin) in-cis,JAM-C forms an intercellular barrier complex that restricts paracellularpermeability. In mouse, however, thisapplication may not apply, as endothelium, rather than epithelium, appears tobe site of maximum expression. In thislocale, JAM-C is suggested to regulate neutrophil exodus from the blood, andprovide a barrier against reverse migration back into the vasculature. In any event, binding partners for JAM-C in-trans include JAM-B, Mac-1, alpha x beta 2and JAM-C itself. Cells known toexpress JAM-C in mouse do not necessarily mirror those expressing JAM-C inhuman. Mouse cells known to containJAM-C are limited in type and include endothelium, high endothelial venules,fibroblasts, hematopoietic stem cells, Schwann cells involved in myelination,and neurons of an Aδ (or pain sensing)phenotype. The extracellular domain ofmouse JAM-C shares 96% and 87% aa sequence identity with the extracellulardomains of rat and human JAM-C, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of JAM‑C (CD323) in B16‑F1 Mouse Cell Line by Flow Cytometry. B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse JAM‑C (CD323) APC‑conjugated Monoclonal Antibody (Catalog # FAB7050A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins. |
