| 货号 | IC479A |
| 别名 | MCAF; MCP-1 |
| 反应种属 | Mouse |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human(rh) CCL1, 2, 3,4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,rmCCL1, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12,17, 19, 20, 21, 22, 24, 25, 27, 28, or rrCCL20. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse CCL2/JE/MCP-1 Gln24-Arg96 Accession # P10148 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CCL2/JE/MCP‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with LPS for 48 hours were stained with Rat Anti-Mouse CCL2/JE/MCP‑1 APC-conjugated Monoclonal Antibody (Catalog # IC479A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
