| 货号 | IC2729A |
| 别名 | FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox | 全称 | Nanog Homeobox |
| 反应种属 | Mouse |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79923 (Human); 71950 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Nanog Trp154-Leu262 Accession # Q80Z64 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1‑3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line either untreated (dark orange filled histogram) or treated with retinoic acid for 3 days (light orange filled histogram) was stained with Goat Anti-Mouse Nanog APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2729A) or isotype control antibody (Catalog # IC108A, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
