| 货号 | IC2085G |
| 别名 | brachyury protein; MGC104817; Protein T; T brachyury (mouse) homolog; T brachyury homolog; T, brachyury homolog (mouse); TF; TFT |
| 反应种属 | Human/Mouse |
| 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6862 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Brachyury Ser2-Glu202 Accession # O15178 |
| 生物活性 | Human, Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
