| 货号 | FAB1525A |
| 别名 | CLEC4N; CLEC6A; CLECSF10; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10; C-type lectin domain family 6 member A; C-type lectin domain family 6, member A; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin 2; DECTIN2; dectin-2; Dendritic cell-associated C-type lectin 2 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse Dectin‑2 alpha /CLEC6A alpha Isoform in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human DLEC is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 93978 (Human); 56620 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑2 alpha /CLEC6A Phe43-Leu209 Accession # Q9JKF4 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | Dectin-2 alpha, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 alpha, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3‑6). The CRD of Dectin-2 alpha contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 alpha selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 alpha induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 alpha is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4+CD25+ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 alpha shares 71% aa sequence identity with human and bovine Dectin-2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Dectin‑2/CLEC6A in Mouse Dendritic Cells by Flow Cytometry. Mouse bone marrow-derived dendritic cells were stained with Goat Anti-MouseDectin‑2/CLEC6A alpha Isoform APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1525A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins. |
