Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) A488 MAb (269434) (100 TESTS)
货号：
IC7806G 基本售价：
4808.5 元 规格：
100 Tests
产品信息
概述| 货号 | IC7806G |
| 别名 | ERK1/ERK2 |
全称 | Extracellular Signal-regulated Kinase 1/2 | | 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells)
|
| 目标/特异性 | Detects human, mouse, and rat ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Phosphopeptide containing the ERK1 T202/Y204 site |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Alexa Fluor 488 |
| 背景 | ERK1 and ERK2 (also known as MAPK3 and MAPK1, respectively) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to Insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. The two proteins share 83% amino acid identity, differing mainly at the N- and C-termini. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Airway Inflammation and Remodeling
- Apoptosis Intracellular Kinases
- Chemokine Receptor Intracellular Signaling
- Common gamma Chain Receptor Family
- C-type Lectin Receptors
- GDNF Family
- ICC / IHC Images: ERK1/ERK2
- IL-1 Family
- IL-17 Family
- Interactive Pathway: IL-1 Family Signaling Pathways
- Intracellular Kinases
- Intracellular Kinases in the Akt Pathway
- Intracellular Signaling Molecules in Angiogenesis
- Mini-review: The ERK Signal Transduction Pathway
- Natural Killer T (NKT) Cells
- Neurotrophin/Trk Family
- PDGF Family
- Positive Regulators of the Jak/STAT Pathway
- ROS and Stress Resistance
- TNF Superfamily Regulators
- VEGF Family
|
参考图片Detection of Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (light orange filled histogram) or treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 10 minutes (dark orange filled histogram) were stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC7806G) or isotype control antibody (Catalog # IC105G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
