Human/Mouse/Rat Caveolin-1 Alexa Fluor 488 MAb (Clone 7C8) (100 TESTS)
货号：
IC5736G 基本售价：
4808.5 元 规格：
100 Tests
产品信息
概述| 货号 | IC5736G |
| 别名 | CAV; CAV1; caveolae protein, 22kD; caveolin 1, caveolae protein, 22kDa; cell growth-inhibiting protein 32; MSTP085; VIP21 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Immunocytochemistry(1:10 dilution)
|
| 目标/特异性 | Designed to visualize the expression of Caveolin-1 by fluorescence microscopy for staining cells and tissues. Conjugated Caveolin-1 antibodies are ideal for immunocytochemistry colocalization studies in caveolae. Detects endogenous human, mouse and rat Caveolin-1 in Western blots. |
| 使用方法 | Immunocytochemistry: 1:10 dilution
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 857 (Human); 12389 (Mouse); 25404 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Puified rat adipocyte low density microsomes
Accession # P41350 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Alexa Fluor 488 |
| 背景 | Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% aa sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Actin Stress Fibers and Focal Adhesions During EMT
- Cancer Biomarkers
- Cell to Cell Adhesion Disassembly During EMT
- Focal Adhesions
- ICC / IHC Images: Caveolin-1
- Muscle Stem Cell Markers
- Myogenesis Markers
- Myogenic Precursor Markers
- Phospholipases, Small GTPases, and Other Molecules in the Akt Pathway
|
参考图片Caveolin‑1 in HeLa Human Cell Line. Caveolin‑1 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC5736G) at 1:10 dilution overnight at 4° C and counterstained with Propidium Iodide (red). Specific staining was localized to caveolae. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
