货号 | IC350A |
别名 | chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1 |
反应种属 | Human/Mouse |
应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs. |
使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Downregulation of CXCL12 in mesenchymal stromal cells by TGF? promotes breast cancer metastasis | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human CXCL12/SDF‑1 alpha Lys22-Lys89 Accession # Q6ICW0 |
生物活性 | Human, Mouse |
标记 | Allophycocyanin |
背景 | CXCL12, also known as SDF-1, is a CXC chemokine that interacts with CXCR4. SDF-1 alpha and SDF-1 beta are alternatively spliced isoforms of CXCL12. SDF-1 beta has an additional 4 amino acids at the carboxy-terminus. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF‑1 APC‑conjugated Monoclonal Antibody (Catalog # IC350A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |