| 货号 | IC5436G |
| 别名 | Phosphoprotein NP33, 40S ribosomal protein S6; pS6; ribosomal protein S6; RPS6; S6 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Immunocytochemistry(1:10 dilution) |
| 目标/特异性 | Conjugated Ribosomal Protein S6/RPS6 antibodies are ideal for immunocytochemistry colocalization studies in ribosomes. The unconjugated antibody detects endogenous human, mouse and rat Ribosomal Protein S6/RPS6 in Western blots. |
| 使用方法 | Immunocytochemistry: 1:10 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6194 (Human); 20104 (Mouse); 29304 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Ribosomal Protein S6/RPS6 Met1-Lys249 Accession # P62753 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Alexa Fluor 488 |
| 背景 | 40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Ribosomal Protein S6/RPS6 in HeLa Human Cell Line. Ribosomal Protein S6/RPS6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6/RPS6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC5436G) at 1:10 dilution for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to ribosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
