| 货号 | FAB2154A |
| 别名 | B7h.5; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1; B7S1VCTN1; B7x; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1 | 全称 | B7 Homolog 4 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse B7‑H4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) B7‑1, rmB7‑2, rmB7‑H1, rmB7‑H2, rmB7‑H3, recombinant human (rh) B7‑H3b, rhB7‑H4, or rmPD‑L2 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79679 (Human); 242122 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse B7-H4 Phe29-Pro258 Accession # Q7TSP5 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | B7-H4, also known as VTCN1, B7x and B7S1, is a 50-60 kDa member of the BTN/MOG family of molecules. In mouse, mature B7-H4 is 232 amino acids (aa) in length, and apparently exists as a GPI-linked molecule. This is in contrast to human B7-H4 which is described as being a type I transmembrane protein that also possesses an NLS. There are two V-type Ig-like domains with multiple glycosylation sites. Over aa 29-258, mouse B7-H4 shares 99% and 90% aa sequence indentity with rat and human B7-H4, respectively. Mouse cells known to express B7-H4 include pancreatic islet alpha - and beta -cells, peritoneal macrophages, hepatic stellate cells, dendritic cells and select tumor types. B7-H4 in both mouse and human is known to be solublilized, and to bind to an as-yet unidentified ligand. Although B7-H4 is described as a T cell inactivator, it would appear that it actually fine-tunes an overall downregulation of T cell activity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of B7‑H4 in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse B7‑H4 APC‑conjugated Monoclonal Antibody (Catalog # FAB2154A) or (B) Rat IgG2B Allophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins. |
