Mouse ROBO4 Allophycocyanin MAb (Clone 274914) (100 TESTS)
货号：
FAB50041A 基本售价：
4808.5 元 规格：
100 Tests
产品信息
概述| 货号 | FAB50041A |
| 别名 | ECSM4; FLJ20798; Magic roundabout; MGC133352; MGC133353; MRB; roundabout homolog 4; roundabout homolog 4, magic roundabout (Drosophila) |
全称 | Roundabout Homolog 4 | | 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects mouse ROBO4 in flow cytometry. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 54538 (Human); 74144 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Y3 rat myeloid cell line transfected with mouse ROBO4 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | ROBO4, also known as Magic Roundabout, is a ~150 kDa glycoprotein belonging to the ROBO family. ROBOs are molecular guidance receptors that typically interact with Slit ligands to regulate axon guidance and neuronal migration. Unlike other family members, ROBO4 is mainly restricted to the vascular endothelium. Expression in early hematopoietic progenitors is also reported. Vascular endothelial ROBO4 is expressed at highest levels in during development and vascular remodeling, including tumor angiogenesis. It is proposed to contribute to vascular stability. Consistent with this, endogenous ROBO4 is concentrated in the vascular stalk and sprouts rather than tip cells and appears to protect newly formed blood vessels against VEGF‑induced vascular leak. ROBO4 binding of Slit proteins has been variably reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers. ROBO4 is also variably reported to stimulate or inhibit cell migration or filopodia formation. Effects on cell movement may be mediated through intracellular binding of WASP-, Ras/Rac/Rho-, Mena-, Src-, or Paxillin‑related proteins, all of which affect the cytoskeleton. Recombinant soluble ROBO4 ECD can antagonize endothelial cell migration and in vivoangiogenesis. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Netrins and Receptors
- Slit Ligands and ROBO Receptors
|
参考图片 Detection of ROBO4 in Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP by Flow Cytometry. Jurkat human acute T cell leukemia cell line transfected with mouse ROBO4 and eGFP was stained with either (A) Rat Anti-Mouse ROBO4 APC‑conjugated Monoclonal Antibody (Catalog # FAB50041A) or (B) Rat IgG 2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins. |
