| 货号 | IC2419A |
| 别名 | Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1 | 全称 | Pancreas/Duodenum Homeobox-1 |
| 反应种属 | Human/Mouse |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human and mouse PDX-1 in Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3651 (Human); 18609 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human PDX-1 Ala91-Arg283 Accession # P52945 |
| 生物活性 | Human, Mouse |
| 标记 | Allophycocyanin |
| 背景 | PDX-1 (Pancreatic-duodenal Homeobox Factor-1), also known as IDX-1 and STF-1 is a 31 kDa member of the IPF-1 (Insulin Promoter Factor) subfamily, Antp (antennapedia) family of molecules. Human PDX-1 is 283 amino acids (aa) in length, and although its predicted MW is approximately 30 kDa, it runs anomalously in SDS-PAGE at 40-46 kDa. PDX-1 possesses one transctivation domain (aa 13-73) and one DNA-binding homeodomain (aa 146-205) that contains an NLS between aa 197-203. Phosphorylation is known to occur on Ser61 and Ser269, and methylation is found on Lys131. PDX-1 drives the development of the embryonic pancreas, and post-natally it is found in both beta - and δ (somatostatin) islet cells. This transcription factor complexes with multiple cofactors (Set9; NeuroD1; MafA; HMGA1) in its regulation of beta -cell division and insulin synthesis, and serves principally as an RNA pol II binding modulator. Over aa 91-283, human and mouse PDX-1 share 87% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX‑1/IPF1 APC‑conjugated Monoclonal Antibody (Catalog # IC2419A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules. |
