| 货号 | FAB11201A |
| 别名 | DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor |
| 反应种属 | Human/Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human and mouse Frizzled-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8321 (Human); 14362 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1 Gln1-His248 (Met122Ile) Accession # O70421 |
| 生物活性 | Human, Mouse |
| 标记 | Allophycocyanin |
| 背景 | Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Frizzled‑1 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB11201A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of Frizzled‑1 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Human/Mouse Frizzled‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB11201A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins. |
