| 货号 | IC8028G |
| 别名 | A170; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; ORCA; OSIL; oxidative stress induced like; p60PDB3; p62; p62B; Paget disease of bone 3; PDB3; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Sequestosome 1; sequestosome-1; SQSTM1; Ubiquitin-binding protein p62; ZIP3 | 全称 | Sequestosome 1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Immunocytochemistry |
| 目标/特异性 | p62/SQSTM1 antibodies are ideal for immunocytochemistry colocalization studies in autophagosomes. The unconjugated antibody detects human p62/SQSTM1 in direct ELISAs, and human, mouse and rat p62/SQSTM1 in Western Blots |
| 使用方法 | Immunocytochemistry: 1:10 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8878 (Human); 18412 (Mouse); 113894 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human p62/SQSTM1 Asp368-Leu440 Accession # Q13501 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Alexa Fluor 488 |
| 背景 | SQSTM1 (Sequestrome-1), also known as p62, is a widely expressed, stress-inducible, multifunctional 62 kDa intracellular protein. The 440 amino acid (aa) human SQSTM1 contains multiple adaptor domains that allow interaction with proteins in NGF/NFkB and other signaling pathways (notably TRAF6, atypical protein kinase C family and Src family), polyubiquitin, proteasome subunits and many others. It contains numerous regulatory phosphorylation sites and a dimerization site. SQSTM1 shuttles ubiquitinylated proteins to the proteasome and is important in autophagy and apoptosis. Its dysregulation is associated with Paget’s disease of bone, Parkinson’s and Alzheimer’s diseases, and cancers. Within aa 344-440, which includes the ubiquitin-binding domain, human SQSTM1 shares 100% aa sequence identity with mouse and rat SQSTM1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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p62/SQSTM1 in RAW 264.7 Mouse Cell Line. p62/SQSTM1 was detected in formaldehyde fixed RAW 264.7 mouse monocyte/macrophage cell line treated with LPS using Mouse Anti-Human/Mouse/Rat p62/SQSTM1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8028G) at 1:10 dilution overnight at 4 °C and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
