| 货号 | IC83951R-100UG |
| 别名 | PLZF; PLZFzinc finger and BTB domain-containing protein 16; ZBTB16; zinc finger and BTB domain containing 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; ZNF145; ZNF145Promyelocytic leukemia zinc finger protein | 全称 | Promyelocytic Leukemia Zinc Finger |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects mouse PLZF in direct ELISAs. |
| 使用方法 | Intracellular Staining by Flow Cytometry |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7704 (Human); 235320 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse PLZF Met1-Gln254 Accession # NP_001028496 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 647 |
| 背景 | Mouse PLZF, also known as Zinc finger and BTB domain containing protein 16 (ZBTB-16) PLZF and ZNF145, is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid (aa) protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc finger domain. PLZF forms dimers with RAR alpha and LAZ3 within its zinc finger region. Alternate splice forms exist that are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. PLZF is highly expressed in undifferentiated, multi-potential hematopoietic progenitor cells, with levels declining as cells commit to various lineages. Mouse PLZF shares 98% and 96% aa identity with rat and human PLZF, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of PLZF in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes stimulated with Recombinant Mouse IL‑12 (Catalog # 419-ML)and Recombinant Mouse IL‑18 (Catalog # 9139-IL) for 3 days were stained with Rat Anti-Mouse NKp46/NCR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Rat Anti-Mouse PLZF Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC83951R) or (B) Rat IgG2A Alexa Fluor 647 Isotype Control (Catalog # IC006R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
