| 货号 | FAB7960N-100UG |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CD289 antigen; CD289; TLR9; toll-like receptor 9 | 全称 | Toll-like Receptor 9 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse TLR-9 in direct ELISAs. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 54106 (Human); 81897 (Mouse); 338457 (Rat) |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse TLR9 Leu26-Asp818 Accession # AAK29625 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 700 |
| 背景 | TLR9 (Toll‑like receptor 9), designated CD289, is a member of the TLR family of innate immune receptors that is mainly expressed by colonic epithelium, CD123+ plasmacytoid predendritic cells (pDC), and splenic transitional B cells (1‑9). TLR9 responds to unmethylated DNA CpG motifs that occur mainly in bacteria and viruses (1, 2). Mouse TLR9 cDNA encodes a 1032 amino acid (aa) type I transmembrane glycoprotein with a 793 aa extracellular domain (ECD) that contains 26 leucine‑rich repeats (LRRs, aa 26‑818), and a 193 aa cytoplasmic domain with a TIR sequence that dimerizes with signaling adaptors such as MyD88 (1). The mouse TLR9 ECD shares 87% aa sequence identity with rat and 71‑74% with human, feline, canine, equine, porcine, bovine and ovine TLR9. Predicted splice forms vary at the N‑terminus by initiating either upstream or downstream of the standard site. The full-length 150 kDa form, which is ligand‑binding but nonsignaling, is found in the endoplasmic reticulum. It undergoes accessory protein-mediated translocation either to the cell membrane or to lysosomes (1‑3). TLR9 is cleaved to remove LRR1‑14, producing an 80 kDa signaling fragment within acidic endolysosomes where it encounters microbial CpG DNA rather than self-DNA (2, 10, 11). However, immune complexes of self‑DNA with lupus erythematosus anti‑DNA antibodies can induce TLR9 activation and IFN‑ alpha production in pDC (4). A soluble form also found in endosomes includes all 26 LRRs and negatively regulates active TLR9 (12). Activation of TLR9 contributes to splenocyte proliferation, pDC maturation, macrophage inflammatory cytokine production, Th1 inflammatory responses, NK cell activation and recruitment, B cell surface MHC class II up‑regulation and immunoglobulin production, and generation and maintenance of memory B cells (1, 5‑9). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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