| 货号 | FAB639V-100UG |
| 别名 | ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse EphA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human EphA1, recombinant mouse (rm) EphA3, 4, 6, 7, 8, recombinant rat EphA5, rmEphB1, 2, 3, 4, or 6 is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1969 (Human); 13836 (Mouse) |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse EphA2 Ala22-Ala535 (predicted) Accession # AAA82113 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 405 |
| 背景 | EphA2, also known as Eck, Myk2, and Sek2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA2 has been shown to bind ephrin-A3, ephrin-A1, ephrin-A5, ephrin-A4, and ephrin-A2 (2, 3). The extracellular domains of mouse and human EphA2 share greater than 92% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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