| 货号 | FAB4748G-100UG |
| 别名 | HJ2; JAG2; Jagged 2; Jagged2; protein jagged-2; SER2 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse Jagged 2 in direct ELISAs. In direct ELISAs, no cross-reactivitywith recombinant human (rh) Jagged 1, rhJagged 2, recombinant rat Jagged 1 isobserved. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3714 (Human); 16450 (Mouse); 29147 (Rat) |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Jagged 2 Met27-Leu1084 Accession # Q9QYE5 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | Mouse Jagged 2 is a 150 kDa (1) member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins, and thereby regulate cell fate determinations during development (2-6). It is a type 1 transmembrane protein that is synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signal sequence, a 1059 aa extracellular region, a 21 aa transmembrane region, and a 144 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats, many of which are also sites of calcium binding, and a cysteine-rich region just proximal to the transmembrane segment (3). Mouse Jagged 2 shares 93% and 90% aa sequence identity with rat and human Jagged 2, respectively. Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (3). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (3). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (3). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, gamma δ T cell lineage differentiation, and the establishment of functional natural killer cell lines (4 - 6). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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