| 货号 | FAB46281R-100UG |
| 别名 | 19A; CD2 subset 1; CD2-like receptor activating cytotoxic cells; CD319 antigen; CD319; CRACC; CRACCCD2-like receptor-activating cytotoxic cells; CS119A24 protein; Membrane protein FOAP-12; novel LY9 (lymphocyte antigen 9) like protein; Novel Ly9; Protein 19A; SLAM family member 7; SLAMF7 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse CRACC/SLAMF7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CRACC is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 57823 (Human); 75345 (Mouse) |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse CRACC/SLAMF7 Ala22-Gly224 Accession # Q8BHK6 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 647 |
| 背景 | CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CRACC consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig‑like V-set domain and one Ig‑like C2-set domain, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). Within the ECD, mouse CRACC shares 53% aa sequence identity with human CRACC. It shares 19%‑35% aa sequence identity with comparable regions of other mouse SLAM proteins including 2B4, BLAME, CD2F-10, CD84, CD229, NTB-A, and SLAM/CD150. Additional isoforms of mouse CRACC are distinguished by deletions and/or substitutions in their cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8+ T cells, activated B cells, and mature dendritic cells (4, 5). It interacts homophilically to induce NK, CTL, and B cell activation (4‑7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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