| 货号 | IC72391V-100UG |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CVIL; CVL; Cytovillin 2; Cytovillin; DKFZp762H157; EZR; Ezrin; FLJ26216; p81; VIL2; VIL2MGC1584; villin 2 (ezrin); Villin 2; villin-2 |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human Ezrin in direct ELISAs and detects human, mouse, and rat Ezrin in Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7430 (Human); 22350 (Mouse); 54319 (Rat) |
| 免疫原 | E. coli-derived recombinant human Ezrin Lys438-Arg562 Accession # P15311 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Alexa Fluor 405 |
| 背景 | EZRIN (also Cytovillin, Villin2 and p81) is a founding member of the ERM family, Band 4.1 Superfamily of proteins. Although its predicted MW is 69 kDa, it runs anomalously at 77-82 kDa in SDS-PAGE. ERZIN is expressed by epithelial cells where it serves as a linker between the cell membrane and the actin cytoskeleton. Its presence is particularly strong in microvilli where it helps organize this structure. In addition, ERZIN also organizes microtubules in lymphocytes at or near the immunological synapse by interacting with Glg1. Human EZRIN is 585 amino acids (aa) in length. It contains a band 4.1 homology/FERM domain that binds CD44, ICAM-1, EBP50 and ERM family members (aa 1-295), a central alpha -helical region (aa 296-352), and a C-terminal ERM and actin-binding/FERM C domain (aa 353-586). EZRIN exists as either a monomer, or a homo/heterodimer. EZRIN is not constitutively active, but must be phosphorylated and unfolded to bind to cytoplasmic proteins. Over aa 438-562, human EZRIN shares 96% aa identity with mouse EZRIN. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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