| 货号 | IC6184V-100UG |
| 别名 | AIRE; APECED protein; APECED; APECEDAPSI; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune regulator (APECED protein)10APS1AIRE1; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy); Autoimmune Regulator; PGA1 |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects mouse AIRE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AIRE is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 326 (Human); 11634 (Mouse); 294328 (Rat) |
| 免疫原 | E. coli-derived recombinant mouse AIRE Ser476-Ser552 (predicted) Accession # Q9Z0E3 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 405 |
| 背景 | AutoImmuneREgulator (AIRE) is an approximately 60 kDa nuclear and cytosolic protein that is required for the development of T cell tolerance. It regulates the expression of self-antigens by thymic epithelial cells, and mutations in AIRE are causative of the autoimmune disorder, APECED. AIRE regulates gene transcription through interactions with DNA, histone H3, and the nuclear matrix. It contains one HSD domain (aa 1-105), a nuclear localization sequence (aa 113-133), one SAND domain (aa 181-280), and two PHD zinc finger domains (aa 299-340 and aa 434-475). Alternate splicing of human AIRE generates isoforms that lack the HSR and SAND domains and/or the second PHD domain. Within aa 476-552, human AIRE shares 65% and 63% aa sequence identity with mouse and rat AIRE, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|