| 货号 | IC1570V-100UG |
| 别名 | IL-12/IL-35 p35; IL-12A; IL35p35 | 全称 | Interleukin 12 p35 |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3592 (Human); 16159 (Mouse); 84405 (Rat) |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35 Arg23-Ser219 Accession # P29459 |
| 生物活性 | Human, Mouse |
| 标记 | Alexa Fluor 405 |
| 背景 | Interleukin12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 iscomprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+regulatory T cells and may function as an inhibitory cytokine to suppress Tcell proliferation (2). Human FoxP3+ Tregs do not constitutivelyexpress IL-35 (3), but expression may be induced by activated dendritic cells(4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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