货号 | IC1368V-100UG |
别名 | AFP; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP |
应用 | Intracellular Staining by Flow Cytometry |
目标/特异性 | Detects human alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots. |
使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 174 (Human); 11576 (Mouse); 24177 (Rat) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP |
生物活性 | Human, Mouse |
标记 | Alexa Fluor 405 |
背景 | alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of a-Fetoprotein/AFP in Human HepG2Cell Line by Flow Cytometry. HepG2 Human hepatocellular carcinomacell line was stained with Mouse Anti-Human/Mouse a-Fetoprotein/AFP Alexa Fluor® 405-conjugatedMonoclonal Antibody (Catalog # IC1368V, filled histogram) or isotype controlantibody (Catalog # IC002V, open histogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for Staining Intracellular Molecules. |