| 货号 | FAB5795U-100UG |
| 别名 | 5 nucleotidase (CD73); 5-NT; 5-NT; 5Nucleotidase; 5-Nucleotidase; 5-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5-nucleotidase; eN; eNT; NT; NT55-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects recombinant human 5-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5-Nucleotidase is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4907 (Human); 23959 (Mouse); 58813 (Rat) |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human 5‑Nucleotidase/CD73 Trp27-Ile511 Accession # P21589 |
| 生物活性 | Human, Equine |
| 标记 | Alexa Fluor 350 |
| 背景 | 5-Nucleotidase (also [ecto]-5-nucleotidase/5-NT, designated CD73) is a variably glycosylated, 69-73 kDa member of the 5-Nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3+ Treg lymphocytes, FDCs and B cells. 5-Nucleotidase hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human 5-Nucleotidase is a 523 amino acid (aa) GPI-linked protein (aa 27-549). It contains a large Zn‑dependent nucleotidase catalytic region (aa 28-532) and a C-terminal substrate binding site (aa 500-506). On the cell surface it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa 405-454, and a second possesses a 12 aa substitution for aa 253-574. Over aa 1-511, human 5‑Nucleotidase shares 88% aa identity with both mouse and rat 5-Nucleotidase. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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