| 货号 | FAB17942R-100UG |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHT; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT | 全称 | TNF Ligand Superfamily Member 14 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Stains mouse LIGHT/TNFSF14 transfectants but not irrelevant transfectants in flow cytometry. |
| 使用方法 | Flow Cytometry: 0.1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8740 (Human); 50930 (Mouse) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse LIGHT Asp72-Val239 Accession # Q9QYH9 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 647 |
| 背景 | LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) is a member of the TNF superfamily and is designated TNFSF14. The gene for mouse LIGHT encodes a 239 amino acid residue (aa) type II transmembrane glycoprotein that contains a 37 aa N-terminal cytoplasmic domain, a 21 aa transmembrane region, and a 181 aa extracellular domain. A soluble form of mouse LIGHT is generated from the membrane form by proteolytic processing. Similar to other TNF ligand family members, LIGHT is assembled as a homotrimer. Mouse and human LIGHT share 71% aa sequence identity. LIGHT is expressed by activated lymphocytes, natural killer cells, immature dendritic cells, monocytes and granulocytes. Mouse LIGHT binds and signals via two distinct TNF receptor superfamily members, including the herpes virus entry mediator (HVEM/TNFRSF14) and the lymphotoxin beta receptor (LT beta R/TNFRSF3). In humans, LIGHT also binds the soluble human decoy receptor 3 (DcR3/TNFRSF6B). Signaling from LT beta R, which also binds LT alpha beta, induces apoptosis and the production of various cytokines. LIGHT-LT beta R signaling also plays a role in mesenteric lymph node organogenesis, and restoration of secondary lymphoid structure and function. Signaling from HVEM, which also binds LT alpha, co-stimulates T-helper cell type 1 (TH1) immune responses, enhances Cytotoxic T Lymphocytes (CTL)-mediated tumor immunity, and regulates allogeneic T cell activation and allograft rejection. Blockade of LIGHT-HVEM signaling has been shown to prevent graft versus host disease. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of LIGHT/TNFSF14 in HEK293 Human Cell Line Transfected with Mouse LIGHT/TNFSF14 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse LIGHT/TNFSF14 or (B) irrelelvant transfectants and eGFP was stained with Rat Anti-Mouse LIGHT/TNFSF14 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB17942R). Quadrant markers were set based on control antibody staining (Catalog # IC006R). View our protocol for Staining Membrane-associated Proteins. |
