Mouse IL-22BP Allophycocyanin Affinity Purified PAb (100 TESTS)【科瑞奥博】

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Mouse IL-22BP Allophycocyanin Affinity Purified PAb (100 TESTS)

货号： IC2376A 基本售价： 4659.8 元规格： 100 Tests

产品信息

概述
货号	IC2376A
别名	class II cytokine receptor; CRF2-10; CRF2-S1IL22BP; CRF2-X; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; Cytokine receptor family type 2, soluble 1; IL-22 RA2; IL-22 receptor subunit alpha-2; IL-22BPCRF2X; IL22RA2; IL-22RA2; IL-22R-alpha-2; interleukin 22 receptor, alpha 2; interleukin 22-binding protein; interleukin-22 receptor subunit alpha-2; Interleukin-22-binding protein; MGC150509; MGC150510; zcytoR16
全称	Interleukin 22 Binding Protein
反应种属	Mouse
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects mouse IL-22BP in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) IL-22BP is observed and 5% cross-reactivity with rhIL-22 R is observed.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	116379 (Human)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL‑22BP Thr19-Pro230 Accession # AAI24999
生物活性	Mouse
标记	Allophycocyanin
背景	Interleukin 22 Binding Protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35‑45 kDa secreted glycoprotein belonging to the Type II Cytokine Receptor Family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is also a component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29 (1-4). IL-10 R beta is widely expressed, and other cell types express either IL-10 R alpha or IL-22 R but not both (5). The mouse IL-22BP cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence and two fibronectin type-III domains (6). Mouse IL-22BP shares 59% and 85% aa sequence identity with human and rat IL-22BP, respectively. Among type II cytokine receptors, IL-22BP is most closely related to the extracellular domain of IL-22 R, sharing 36% aa sequence identity. Splice variants of human IL-22BP exist but have not been detected for the mouse protein (6-8). IL-22BP binds an epitope on IL-22 that is required for the interaction of IL-22 with the transmembrane IL-22 R (8-10), thereby preventing the association of IL-22 R with IL-10 R beta (10). IL-22BP blocks IL-22-induced responses including induction of MHC class I expression, Stat1 and Stat3 activation, SOCS3 gene expression,the production of reactive oxygen species (6, 9) and the modulation of the proinflammatory effects of IL-22 (11). Mouse IL-22 BP neutralizes both mouse and human IL-22 (6). IL-22BP is secreted by epithelial cells, dendritic cells, activated B cells, and activated monocytes (6, 8, 12, 13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. Kotenko, S.V. (2002) Cytokine Growth Factor Rev. 13:223. Wolk, K. et al. (2005) Genes Immun. 6:8. Wei, C.C. et al. (2003) Genes Immun. 4:204. Dumoutier, L. et al. (2001) J. Immunol. 166:7090. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096. Li, J. et al. (2004) Int. Immunopharmacol. 4:693. Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220. Lecart, S. et al. (2002) Int. Immunol. 14:1351. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.

参考图片

Detection of IL‑22BP in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse IL‑22BP APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2376A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.