| 货号 | FAB3328P |
| 别名 | CD223 antigen; CD223; FDC; LAG-3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC | 全称 | Lymphocyte-activation Gene 3 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse LAG-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human LAG-3 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse LAG-3 Gly24-Leu442 Accession # Q61790 |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | LAG-3 (Lymphocyte Activation Gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity. The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of LAG‑3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with PMA and Calcium Ionomycin for 3 days were stained with Goat Anti-Mouse LAG‑3 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3328P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
