货号 | FAB1434G |
别名 | Stage specific embryonic antigen 3; Stage-specific embryonic antigen 3 | 全称 | Stage-specific Embryonic Antigen-3 |
反应种属 | Human/Mouse |
应用 | Flow Cytometry(5 µL/106cells) |
目标/特异性 | Recognizes a carbohydrate epitope of SSEA-3 (4, 5). |
使用方法 | Flow Cytometry: 5 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
纯化方式 | IgM-specific Affinity-purified from hybridoma culture supernatant |
免疫原 | Four to eight cell stage mouse embryos |
生物活性 | Human, Mouse |
标记 | Alexa Fluor 488 |
背景 | SSEA-3, also known as glycolipid GB5, is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES) (1). Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression (2, 3). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of SSEA‑3 in BG01V Human Embryonic Stem Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Rat Anti-Human/Mouse SSEA‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1434G, filled histogram) or isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of SSEA‑3 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Rat Anti-Human/Mouse SSEA‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1434G, filled histogram) or isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins. |