Human/Mouse Myogenin Alexa Fluor 488 MAb (Clone 671038) (100 TESTS)【科瑞奥博】

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Human/Mouse Myogenin Alexa Fluor 488 MAb (Clone 671038) (100 TESTS)

货号： IC6686G 基本售价： 4659.8 元规格： 100 Tests

产品信息

概述
货号	IC6686G
别名	BHLHC3; bHLHc3Myogenic factor-4; myogenin; Class C basic helix-loop-helix protein 3; MYF4; myf-4; MYF4myogenin; MYOG; Myogenic factor 4; myogenin (myogenic factor 4); MYOGENIN
反应种属	Human/Mouse
应用	Intracellular Staining by Flow Cytometry(5 µL/10⁶cells)
目标/特异性	Detects human Myogenin in direct ELISAs and mouse Myogenin in flow cytometry.
使用方法	Intracellular Staining by Flow Cytometry: 5 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4656 (Human); 17928 (Mouse); 29148 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Platelet-rich plasma, especially when combined with a TGF-beta inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro. Authors: Kelc R, Trapecar M, Gradisnik L, Rupnik M, Vogrin M PLoS ONE, 2015;10(2):e0117302. Species: Human Sample Type: Whole Cells Application: Flow Mesenchymal Stem Cells from Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project. Authors: Boyle K, Patinkin Z, Shapiro A, Baker P, Dabelea D, Friedman J Diabetes, 0;0(0):. Species: Human Sample Type: Cell Culture Supernates Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Myogenin Ser124-Asn224 Accession # P15173
生物活性	Human, Mouse
标记	Alexa Fluor 488
背景	Myogenin, also known as MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the Myogenic Regulatory Factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa sequence identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.
运输条件	Blue Ice
存放说明	4℃
参考文献	Basic Helix-Loop-Helix (bHLH) Transcription Factors ICC / IHC Images: Myogenin Mesenchymal Stem Cell Transcription Factors Muscle Stem Cell Markers Myogenesis Markers Myogenic Precursor Markers Skeletal Muscle Markers Somite Markers Transcription Factors in the Akt Pathway

参考图片

Detection of Myogenin in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse Myogenin Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6686G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.