Mouse/Rat Nestin Phycoerythrin MAb (Clone 307501) (100 TESTS)【科瑞奥博】

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Mouse/Rat Nestin Phycoerythrin MAb (Clone 307501) (100 TESTS)

货号： IC2736P 基本售价： 4659.8 元规格： 100 Tests

产品信息

概述
货号	IC2736P
别名	FLJ21841; NES; nestin
反应种属	Mouse/Rat
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects mouse and rat Nestin in Western blots.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10763 (Human); 18008 (Mouse); 25491 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile J Immunol, 2016;196(11):4760-70. Species: Mouse Sample Type: Whole Cells Application: Flow Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma. Authors: Hu S, Rotschafer J, Lokensgard J, Cheeran M PLoS ONE, 2014;9(8):e105219. Species: Mouse Sample Type: Whole Cells Application: Flow Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain. Authors: Mutnal MB, Cheeran MC, Hu S PLoS ONE, 2011;6(1):e16211. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant rat Nestin Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys) Accession # EDM00749
生物活性	Mouse, Rat
标记	Phycoerythrin
背景	Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14). Over amino acids (aa) 544-820, rat Nestin shares 75% and 38% sequence identity with mouse and human Nestin, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310. Lendahl, U. et al. (1990) Cell 60:585. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144. Tohyama, T. et al. (1992) Lab. Invest. 66:303. Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720. Frederiksen, K. et al. (1988) Neuron 1:439. Cattaneo, E. et al. (1990) Nature 347:762. Reynolds, B.A. and S. Weiss (1992) Science 255:1707. Rietze, R.L. et al. (2001) Nature 412:736. Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383. Zulewski, H. et al. (2001) Diabetes 50:521. Lumelsky, N. et al. (2001) Science 292:1389. Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670. Shih, C.C. et al. (2001) Blood 98:2412.

参考图片

Detection of Nestin in Mouse Cortical Stem Cells by Flow Cytometry. Mouse cortical stem cells were stained with Mouse Anti-Mouse/Rat Nestin PE‑conjugated Monoclonal Antibody (Catalog # IC2736P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Nestin in Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells were stained with Mouse Anti-Mouse/Rat Nestin PE‑conjugated Monoclonal Antibody (Catalog # IC2736P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules.