| 货号 | FAB1817A |
| 别名 | CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1 | 全称 | T Cell Immunoglobulin Mucin-1 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse TIM‑1/KIM-1/HAVCR in ELISAs and Western blots. In sandwich immunoassays, this antibody does not cross-react with recombinant human (rh) TIM‑1, recombinant mouse (rm)TIM-2, or rmTIM-3. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR Tyr22-Thr212 Accession # NP_001160104 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | TIM-1 (T cell-Immunoglobulin-Mucin; also known as KIM-1 or HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39% and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of TIM‑1/KIM‑1/HAVCR in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 200 ng/mL LPS for 16 hours were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse TIM‑1/KIM‑1/HAVCR APC‑conjugated Monoclonal Antibody (Catalog # FAB1817A) or (B) Rat IgG2B Allophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins. |
