Mouse Rae-1 epsilon Allophycocyanin MAb (Clone 205001) (100 TESTS)
货号：
FAB1135A 基本售价：
4659.8 元 规格：
100 TESTS
产品信息
概述| 货号 | FAB1135A |
| 简介 | FAB1135A has been discontinued. |
| 别名 | Rae1 epsilon; Rae-1 epsilon |
全称 | Retinoic Acid Early Transcript 1 epsilon | | 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects mouse Rae‑1 epsilon in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Rae-1 alpha, beta, gamma, or δ is observed. By flow cytometry, no cross-reactivity with mouse Rae-1 alpha or mouse Rae-1 gamma. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 8480 (Human); 379043 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | BaF3 mouse pro-B cell line transfected with mouse Rae-1 epsilon |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | Rae-1 epsilon is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, gamma, and δ. Amino acid sequence identity within this family ranges from 88-95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI-linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10-14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1-7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
- Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
- Cerwenka, A. et al. (2000) Immunity 12:721.
- Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
- Diefenbach, A. et al. (2001) Nature 413:165.
- Champsaur, M. et al. (2010) J. Immunol. 185:157.
- Markiewicz M. et al. (2012) Immunity 36:132.
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参考图片Detection of Rae‑1 epsilon in BaF3 Mouse Cell Line Transfected with Mouse Rae‑1 epsilon by Flow Cytometry. BaF3 mouse pro-B cell line transfected with mouse Rae‑1 epsilon (top) or mouse Rae-1 gamma (bottom, negative control) was stained with Rat Anti-Mouse Rae‑1 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB1135A). Quadrant markers were set based on control antibody staining (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins. |
