| 货号 | FAB4488A |
| 别名 | 5 nucleotidase (CD73); 5-NT; 5-NT; 5-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5-nucleotidase; eN; eNT; NT; NT55-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse 5‑Nucleotidase/CD73 in direct ELISAs. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4907 (Human); 23959 (Mouse); 58813 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse 5‑Nucleotidase/CD73 Trp29-Lys549 Accession # Q61503 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of 5‑Nucleotidase/CD73 in Mouse splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse 5‑Nucleotidase/CD73 APC‑conjugated Monoclonal Antibody (Catalog # FAB4488A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins. |
