Mouse CCR8 Allophycocyanin MAb (Clone 1055c) (100 TESTS)
货号：
FAB8324A-100 基本售价：
4659.8 元 规格：
100 Tests
产品信息
概述| 货号 | FAB8324A-100 |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CC chemokine receptor 8; C-C CKR-8; CC-chemokine receptor chemr1; CC-CKR-8; CCR8; CCR-8; CD198; CDw198 antigen; CDw198; chemokine (C-C motif) receptor 8; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; Chemokine receptor-like 1; ChemR1; CKRL1; CKR-L1; CKRL1MGC129966; CMKBR8MGC129973; CMKBRL2C-C chemokine receptor type 8; CY6; GPRCL1; GPRCY6; GPR-CY6; TER-1; TER1CC chemokine receptor CHEMR1 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Stains mouse CCR8 transfectants but not irrelevant transfectants in flow cytometry. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 1237 (Human); 12776 (Mouse) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | HEK293 human embryonic kidney cell line transfected with mouse CCR8
Accession # NP_031746 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | CCR8 (C-C chemokine Receptor 8; also known as CD198) is a 41-43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Mouse CCR8 is a 7-transmembrane protein that is 353 amino acids (aa) in length. It contains a 33 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. In mouse, CCR8 is N- and possibly O-glycosylated, and known to be sulfated on Tyr14 and 15. The unusual nature of these posttranslational modifications may lead to anomalous migration in SDS-PAGE. There are two potential isoforms, one that shows a deletion of aa 103-163, and another that shows a Met substitution for aa 125-166. Over aa sequences 1-33 and 92-105 collectively, mouse CCR8 shares 64% and 85% aa identity with human and rat CCR8, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - CC Chemokines and Receptors
- Chemokine Receptors
- Chemokines and Receptors in Allergy and Asthma
- Chemokines and Receptors in Angiogenesis
- Human CD Marker Products
- Monocyte Markers
- Regulatory T Cells (Tregs)
- T Cell Migration/Adhesion
- Technical Notes: Antibodies to Complex Antigens
- Th2 Cells
- Thymocytes
|
参考图片Detection of CCR8 in HEK293 Human Cell Line Transfected with Mouse CCR8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse CCR8 and eGFP (top panel) or irrelevant transfectant (bottom panel) were stained with Rabbit Anti-Mouse CCR8 APC‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # FAB8324A). Quadrant markers were set based on control antibody staining (Catalog # IC105A). View our protocol for Staining Membrane-associated Proteins. |
