| 货号 | FAB7681A |
| 别名 | CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; FLT-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3 | 全称 | fms-like Tyrosine Kinase 3 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse Flt‑3/Flk‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Flt-3 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2322 (Human); 14255 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Flt‑3/Flk‑2 Asn28-Ser544 Accession # Q00342 |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (Fetal Liver Kinase) and Stk-1 (Stem cell Tyrosine Kinase) and designated CD135, is a 150 kDa member of the class III subfamily of receptor tyrosine kinases. This familay includes KIT, the receptor for SCF, and C-FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Mouse Flt-3 cDNA encodes a 992 amino acid (aa) residue type I membrane protein with a 27 aa residue signal peptide, a 517 aa extracellular domain with 10 potential N-linked glycosylation sites, a 20 aa residue transmembrane domain and a 428 aa residue cytoplasmic domain. Mouse Flt-3 shares 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected on neurons and hematopoietic progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3 Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist. Over aa 28-544, mouse Flt-3 shares 89% and 84% aa sequence identity with rat and human Flt-3, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Flt‑3/Flk‑2 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Rat Anti-Mouse Flt‑3/Flk‑2 APC‑conjugated Monoclonal Antibody (Catalog # FAB7681A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins. |
