| 货号 | FAB5375G |
| 别名 | CD232 antigen; CD232; plexin (semaphorin receptor); plexin C1; PLXNC1; PLXN-C1; receptor for viral semaphorin protein; receptor for virally-encoded semaphorin; VESPR; VESPRplexin-C1; Virus-encoded semaphorin protein receptor |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects mouse Plexin C1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse Plexin A1, A2, A3, recombinant human Plexin B1, B2, and D1 is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10154 (Human); 54712 (Mouse); 362873 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin C1 Ala35-Thr950 Accession # Q9QZC2 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | Plexin C1 (also VESPR and CD232) is a 200‑210 kDa member of the plexin C subfamily, plexin family of proteins. It is a type I transmembrane glycoprotein that is found on monocytes, dendritic cells, neutrophils and embryonic neuroendocrine neurons of the hyypothalamus. Plexin C1 binds Sema7A, and its viral counterpart, vaccinia semaphorin A39R. Ligation induces actin cytoskeletal rearrangement and cell immobilization. Mature mouse Plexin C1 is 1540 amino acids (aa) in length. It contains a 916 aa extracellular domain that shows one Sema-domain (aa 35‑452) and a 603 aa cytoplasmic tail. There are two potential splice variants that show a five and 67 aa substitution for aa 861‑870 and 1206‑1574, respectively. Over aa 35‑950, mouse Plexin C1 is 95% and 85% aa identical to rat and human Plexin C1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Plexin C1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse Plexin C1 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5375G, filled histogram) or isotype control antibody (Catalog # IC016G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
