| 货号 | IC3535G |
| 别名 | Ecalectin; GAL9; gal-9; galectin 9; galectin-9; HUAT; lectin, galactoside-binding, soluble, 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; urate transporter/channel protein |
| 反应种属 | Mouse |
| 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects mouse Galectin-9 in direct ELISAs. In direct ELISAs, no cross-reactivitywith recombinant human Galectin-9 or recombinant mouse Galectin-4 is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3965 (Human); 16859 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse Galectin-9 (short isoform) Ala2-Thr322 Accession # O08573-2 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | Mouse Galectin-9 ( beta -Galactoside-binding Lectin 9) is a 36-39 kDa, secreted, S-type lectin. It is 353 amino acids (aa) in length and contains no identifiable signal sequence. There are two distinct carbohydrate-binding regions (aa 81-87 and 285-291) that are joined by a linker region (aa 148-205). At least one alternate splice form exists that shows a 31 aa insertion between aa 147-148. The short form is expressed on fibroblasts, hepatocytes, endothelial cells and astrocytes. The long form is expressed on intestinal epithelium. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 is 69% and 85% aa identical to human and rat Galectin-9, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Galectin‑9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse Galectin‑9 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC3535G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
