| 货号 | FAB62291G |
| 别名 | D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32 | 全称 | Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects mouse LRRC32/GARP in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivitywith recombinant human (rh) LRRC32 is observed and no cross-reactivity withrhLRRC3, rhLRRC4, or rhNGL-3/LRRC4B is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2615 (Human); 434215 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse LRRC32/GARP Ile18-Asn628 Accession # NP_001106850 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | Leucine-rich Repeat Protein 32 (LRRC32), also known as GARP (Glycoprotein A Repetitions Predominant), is an 77-80 kDa type I transmembrane glycoprotein. Mature mouse LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine-rich repeats, followed by a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain. Within the ECD, mouse LRRC32 shares 80 and 94% aa sequence identity with human and rat LRRC32, respectively. LRRC32 is expressed on hepatic stellate cells and on adult platelets. Among T cells, it is selectively expressed on activated FOXP3+ regulatory T cells (Treg). LRRC32 expression promotes the acquisition of a Treg phenotype that is characterized by reduced cellular proliferation and cytokine secretion, plus the capacity to suppress the proliferation of naïve T cells. LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and, in association with alpha V beta 8 Integrin, mediates the activation and release of TGF-beta from the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of LRRC32/GARP in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stimulated with anti-CD3/anti-CD28, Mouse Recombinant IL-2 (Catalog # 402-ML), and TGF-beta for 3 days to induce regulatory T cell differentiation. Cells were stained with Rat Anti-Mouse CD4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB554C) and either (A) Rat Anti-Mouse LRRC32/GARP Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB62291G) or (B) Rat IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC005G). View our protocol for Staining Membrane-associated Proteins. |
