| 货号 | IC6816G |
| 别名 | bA325O24.3; bA325O24.4; C6orf190; C6orf207; FLJ40584; Gasp; GRB2-associated protein; MGC163388; protein THEMIS; signaling phosphoprotein specific for T cells; SPOT; thymocyte selection associated; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; TSEPA | 全称 | Thymocyte-expressed Molecule Involved in Selection |
| 反应种属 | Mouse |
| 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects mouse Themis in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) Themis (aa 2‑282) is observed under non-reducing conditions, and approximately 10% cross-reactivity with rhThemis (aa 2‑282) is observed under reducing conditions. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 387357 (Human); 210757 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse Themis Lys122-Lys237 Accession # Q8BGW0 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | Themis (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid (aa) cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling. Within the region used as an immunogen, mouse Themis shares 87% and 96% aa sequence identity with human and rat Themis, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Themis in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse Themis Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6816G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
