Mouse Sca-1 Alexa Fluor 488 MAb (Clone 177228) (100 TESTS)【科瑞奥博】

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Mouse Sca-1 Alexa Fluor 488 MAb (Clone 177228) (100 TESTS)

货号： FAB1226G 基本售价： 4521.0 元规格： 100 Tests

产品信息

概述
货号	FAB1226G
别名	Ly6
全称	Stem Cell Antigen-1/Lymphocyte Antigen 6
反应种属	Mouse
应用	Flow Cytometry
目标/特异性	Detects mouse Sca‑1/Ly6 in direct ELISAs.
使用方法	Flow Cytometry: 5 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	110454 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1/Ly6 C-terminally truncated Ly-6E allele Leu27-Gly119 Accession # CAA28351
生物活性	Mouse
标记	Alexa Fluor 488
背景	Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12). Over amino acids (aa) 27-119, mouse Sca-1 shares 58% aa identity with rat Ly6B.
运输条件	Blue Ice
存放说明	4℃
参考文献	Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634. Spangrude, G.I. et al. (1988) Science 241:58. Spangrude, G.I. et al. (1993) Blood 82:3327. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011. Yamamoto, Y. et al. (1996) Blood 88:445. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908. Welm, B.E. et al. (2002) Dev. Biol. 245:42. Codias, E.K. et al. (1990) J. Immunol. 144:2197. Malek, T.R. et al. (1986) J. Exp. Med. 164:709. Codias, E.K. et al. (1990) J. Immunol. 145:1407. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.

参考图片

Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit APC‑conjugated Monoclonal Antibody (Catalog # FAB1356A) and either (A) Rat Anti-Mouse Sca‑1/Ly6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1226G) or (B) Rat IgG_2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.