| 货号 | IC421F |
| 别名 | CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A | 全称 | Interleukin 17 |
| 反应种属 | Mouse |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse IL-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL‑17D, and rmIL-17E is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse IL‑17 Thr22-Ala158 Accession # Q62386 |
| 生物活性 | Mouse |
| 标记 | Fluorescein |
| 背景 | Interleukin 17, also known as IL-17A and CTLA-8, was initially identified as a 17 kDa, secreted T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 25 amino acid residue signal peptide that is cleaved to yield the 133 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 is also known to form a heterodimer with IL-17F. At the amino acid level, mIL-17 shows 62% and 87% aa sequence identity with human and rat IL-17, respectively. The receptor for the IL-17A homodimer and IL-17A:F heterodimer is reported to be a combinationof IL-17 RA and IL-17 RC, with a possible contribution by IL-17 RD. The expression of IL-17 is widespread, and found associated with LTi cells, B cells, gamma δ T cells, CD4+ Th17 cells, iNKT cells, neutrophils, intestinal Paneth cells, Type I ILCs and CD8+Tc17 cells. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation, the preservation of intestinal mucosal integrity, and the induction of antimicrobial peptides by epithelium. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑17/IL‑17A in Activated EL‑4 Mouse Cell Line by Flow Cytometry. Activated EL‑4 mouse lymphoblast cell line was stained with Goat Anti-Mouse IL‑17/IL‑17A Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC421F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
